Journal: World Journal of Stem Cells

Article Title: Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential

doi: 10.4252/wjsc.v16.i5.512

Figure Lengend Snippet: Generation and identification of the human induced pluripotent stem cells derived from the retinitis pigmentosa patient. A: Timeline of human induced pluripotent stem cell generation; B: Imaging by phase-contrast microscopy. Scale bar = 200 μm; C: Karyotype analysis of the healthy control (left) and retinitis pigmentosa patient (right); D: Flow cytometry of pluripotency markers SSEA-4 and TRA-1-81; E and F: Immunostaining of pluripotency markers OCT4, NANOG, SOX2, and SSEA4 of the healthy control and retinitis pigmentosa patient. Scale bar = 20 μm; G and H: In vitro differentiation of control (G) and patient (H) induced pluripotent stem cells into three germ layers, endoderm (AFP+), mesoderm (α-SMA+) and ectoderm (GFAP+). Scale bar = 20 μm. PB: Peripheral blood; PBMC: Peripheral blood mononuclear cell; iPSC: Induced pluripotent stem cell.

Article Snippet: Following primary antibodies were used: OCT4 (Cat. #ab18976; Abcam), SOX2 (Cat. #sc-365823; Santa Cruz), NANOG (Cat. #ab80892; Abcam), SSEA4 (Cat. #ab16287; Abcam), GFAP (Cat. #HPA056030; Sigma), α-SMA (Cat. #A5228; Sigma), AFP (Cat. #MAB1368; R&D System).

Techniques: Derivative Assay, Imaging, Microscopy, Control, Flow Cytometry, Immunostaining, In Vitro

Journal: Cell reports

Article Title: Critical roles of translation initiation and RNA uridylation in endogenous retroviral expression and neural differentiation in pluripotent stem cells

doi: 10.1016/j.celrep.2020.107715

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies and dilution rate were as follows; mouse monoclonal anti-OCT3/4 (1:200, sc-5279, Santa Cruz Biotechnology), rabbit polyclonal anti-SOX2 (1:100, ab97959, Abcam), rabbit polyclonal anti-NANOG (1:100, ab21624, Abcam), mouse monoclonal anti-human Nuclei (1:1000, MAB4383, EMD Millipore), rabbit polyclonal anti-AFP (1:200, A0008, DAKO), mouse monoclonal anti-AFP (1:200, MAB1368, R&D Systems), mouse monoclonal anti-SMA (1:100, M0851, DAKO), mouse monoclonal anti-βIII-TUBULIN (1:1000, MAB1637, EMD Millipore), rabbit polyclonal anti-PAX6 (1:1000, 901301, BioLegend), rabbit polyclonal anti-KLF4 (1:100, sc-20691, Santa Cruz Biotechnology), goat polyclonal anti-TFCP2L1 (1:100, AF5276, R&D Systems), rabbit polyclonal anti-TFE3 (1:100, HPA023881, Sigma-Aldrich), Alexa 488-conjugated donkey anti-mouse IgG (1:500, A-21202, Thermo Fisher Scientific), Alexa 555-conjugated donkey anti-rabbit IgG (1:500, A-31572, Thermo Fisher Scientific), Alexa 647-conjugated donkey anti-mouse IgG (1:500, A-31571, Thermo Fisher Scientific), Alexa 488-conjugated anti-rabbit IgG (1:500, A-21206, Thermo Fisher Scientific), Alexa 555-conjugated donkey anti-rabbit IgG (1:500, A-31572, Thermo Fisher Scientific), Alexa 647-conjugated donkey anti-rabbit IgG (1:500, A-31573, Thermo Fisher Scientific) and Alexa 488-conjugated donkey anti-goat IgG (1:500, A-11055, Thermo Fisher Scientific).

Techniques: Recombinant, Protease Inhibitor, Modification, Knock-Out, Transfection, Blocking Assay, Western Blot, Immunocytochemistry, Lysis, Purification, Clone Assay, SYBR Green Assay, Reporter Assay, TaqMan Assay, Expressing, Microarray, shRNA, Plasmid Preparation, Software

Journal: Journal of Translational Medicine

Article Title: Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor

doi: 10.1186/1479-5876-10-46

Figure Lengend Snippet: Histogram of the expressions of alpha- fetoprotein (A), placental alkaline phosphatase (B) and cytokeratins (C) in the tissue formed from cloned testicular yolk sac tumor cells and stained by Immunohistochemistry . Chromosomes with the diploid structure in cloned testicular yolk sac tumor cells ( D ). The origin magnification was × 400.

Article Snippet: Sections were digested with 0.25% pepsin (Sigma) dissolved in 0.1 M HCl for 15 minutes at 37°C, blocked for 30 minutes in PBS containing 5% normal mouse serum (Jackson Immunoresearch, Newmarket, UK), and then incubated with antibodies again alpha-fetoprotein (AFP, goat anti-mouse alpha-fetoprotein polyclonal antibody; R&D Systems, Minneapolis, USA), placental alkaline phosphatase (PLAP, anti-placental alkaline phosphatase antibody, Abcam, Cambridge, UK), or cytokeratins (CK, mouse anti-mouse cytokeratin 10 monoclonal antibody, unconjugated, clone Spm262; Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours, while HRP-conjugated secondary antibodies for 30 minutes, both at room temperature.

Techniques: Clone Assay, Staining, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor

doi: 10.1186/1479-5876-10-46

Figure Lengend Snippet: Cloned testicular yolk sac tumor cell growth curve during 8 day culture (A), with some abnormal chromosomes (B, X400), or positive expression of alpha- fetoprotein (C, × 200), rather than beta-subunit human chorionic gonadotrophin (D, × 200) .

Article Snippet: Sections were digested with 0.25% pepsin (Sigma) dissolved in 0.1 M HCl for 15 minutes at 37°C, blocked for 30 minutes in PBS containing 5% normal mouse serum (Jackson Immunoresearch, Newmarket, UK), and then incubated with antibodies again alpha-fetoprotein (AFP, goat anti-mouse alpha-fetoprotein polyclonal antibody; R&D Systems, Minneapolis, USA), placental alkaline phosphatase (PLAP, anti-placental alkaline phosphatase antibody, Abcam, Cambridge, UK), or cytokeratins (CK, mouse anti-mouse cytokeratin 10 monoclonal antibody, unconjugated, clone Spm262; Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours, while HRP-conjugated secondary antibodies for 30 minutes, both at room temperature.

Techniques: Clone Assay, Expressing

Journal: Journal of Translational Medicine

Article Title: Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor

doi: 10.1186/1479-5876-10-46

Figure Lengend Snippet: All closed cells used for the measurement of cisplatin effects were alpha- fetoprotein -stained positive (A1) . Apoptotic cells were detected with AO/EB staining (A2). Increased TUNEL-positive cells were detected 12 hours after the treatment with cisplatin (A4) or vehicle (A3). The expression of p53 and Bcl-2 proteins was altered 12 hours and on after the treatment with vehicle (B1 and B3) or cisplantin (B2 and B4), respectively. Values of optimal density of p53 (C) and Bcl-2 protein staining (D) were calculated during 12 and 72 hours after cisplatin treatment.

Article Snippet: Sections were digested with 0.25% pepsin (Sigma) dissolved in 0.1 M HCl for 15 minutes at 37°C, blocked for 30 minutes in PBS containing 5% normal mouse serum (Jackson Immunoresearch, Newmarket, UK), and then incubated with antibodies again alpha-fetoprotein (AFP, goat anti-mouse alpha-fetoprotein polyclonal antibody; R&D Systems, Minneapolis, USA), placental alkaline phosphatase (PLAP, anti-placental alkaline phosphatase antibody, Abcam, Cambridge, UK), or cytokeratins (CK, mouse anti-mouse cytokeratin 10 monoclonal antibody, unconjugated, clone Spm262; Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours, while HRP-conjugated secondary antibodies for 30 minutes, both at room temperature.

Techniques: Staining, TUNEL Assay, Expressing